Preserving Protein Integrity and Post-Translational Modif...

2025-10-21

Translational researchers face mounting challenges in preserving protein structure and post-translational modifications (PTMs) during sample preparation—a critical step for meaningful proteomic and signaling analyses. This thought-leadership article delivers mechanistic insight and practical strategy, anchored in recent discoveries on lactate-driven HMGB1 modifications in sepsis, to justify and elevate the use of advanced, EDTA-free protease and phosphatase inhibitor cocktails. We contextualize the Protease and Phosphatase Inhibitor Cocktail (EDTA Free, 100X in ddH2O) as an essential tool for safeguarding protein integrity, benchmark it against the competitive landscape, and provide a blueprint for its translational application. By integrating the latest research and offering actionable guidance beyond standard product literature, this piece sets a new standard for how inhibitor selection shapes clinical and discovery research outcomes.